The agri-food system, which spans from input supply to food disposal, sustains 2.5 billion livelihoods and feeds the global population. However, it heavily depends on—and simultaneously threatens—biodiversity and ecosystems. Agriculture is the leading driver of deforestation, biodiversity loss, and soil degradation, impacting 86% of species at risk of extinction. Despite these challenges, the sector holds immense potential to drive nature-positive change. To ensure food security, human health, and ecological balance, a rapid transformation in how food is produced, sourced, and consumed is critical. Sector-specific actions guide businesses in supporting the Global Biodiversity Framework’s mission to reverse nature loss by 2030.